Levin D, Constant S, Pasqualini T, Flavell R, Bottomly K
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510.
J Immunol. 1993 Dec 15;151(12):6742-50.
The contribution of dendritic cells (DC) in the initial priming of CD4+ T lymphocytes in vivo was examined. To assess the capacity of different APC to prime CD4+ T cells, a series of MHC class II I-E transgenic mice that differentially express I-E on APC were utilized. Transgenic mice that express I-E primarily on DC, on macrophages, and on B cells were primed with an I-E restricted peptide in vivo, and the extent of priming was determined by restimulation of CD4+ T cells in vitro with the same Ag. The results indicate that DC are required for priming of CD4+ T cells, and that the extent of priming correlates with the frequency of I-E+ DC. Moreover, DC alone can serve as APC for the peptide Ag, in that priming can be restored in an I-E negative mouse by the transfer of peptide-pulsed I-E+ DC. The potency of DC, compared with B cells and macrophages, to prime naive CD4+ T cells was confirmed with in vitro studies.
研究了树突状细胞（DC）在体内对CD4 + T淋巴细胞初始致敏过程中的作用。为了评估不同抗原呈递细胞（APC）致敏CD4 + T细胞的能力，使用了一系列在APC上差异表达I-E的II类MHC I-E转基因小鼠。主要在DC、巨噬细胞和B细胞上表达I-E的转基因小鼠在体内用I-E限制性肽进行致敏，致敏程度通过在体外使用相同抗原再次刺激CD4 + T细胞来确定。结果表明，CD4 + T细胞致敏需要DC，且致敏程度与I-E + DC的频率相关。此外，单独的DC可作为肽抗原的APC，因为通过转移肽脉冲化的I-E + DC可在I-E阴性小鼠中恢复致敏。与B细胞和巨噬细胞相比，DC在体外研究中证实了其致敏初始CD4 + T细胞的能力。
