Bossu P, Singer G G, Andres P, Ettinger R, Marshak-Rothstein A, Abbas A K
Department of Pathology, Brigham and Women's Hospital, Boston, MA.
J Immunol. 1993 Dec 15;151(12):7233-9.
The goal of this study was to examine the functional responses and tolerance susceptibility of T lymphocytes from mice of the autoimmune strain, MRL/lpr. A population of autoreactive CD4+ T cells can be readily expanded from the lymphoid tissues of young lpr mice. Lines of IL-2-producing autoreactive and alloreactive lpr and alloreactive +/+ T cells were developed to study their responses to tolerance-inducing stimuli. Culture of +/+ T cells with high concentrations of immobilized anti-CD3 antibody induces both functional anergy and apoptosis. By contrast, lpr-derived T cell lines are relatively resistant to anergy and apoptosis. The implications of these findings for the development of autoimmunity, and the possible role of the Fas Ag in determining resistance or susceptibility to tolerance, are discussed.
本研究的目的是检测自身免疫性品系MRL/lpr小鼠T淋巴细胞的功能反应和耐受敏感性。可轻易从小鼠幼崽的淋巴组织中扩增出一群自身反应性CD4+ T细胞。为了研究它们对诱导耐受刺激的反应，构建了产生白细胞介素-2的自身反应性、同种异体反应性lpr以及同种异体反应性+/+ T细胞系。用高浓度的固定化抗CD3抗体培养+/+ T细胞会诱导功能性无反应性和细胞凋亡。相比之下，源自lpr的T细胞系对无反应性和细胞凋亡具有相对抗性。讨论了这些发现对自身免疫性疾病发展的意义，以及Fas抗原在决定耐受抗性或敏感性方面可能发挥的作用。
