对HLA-DR3阳性的格雷夫斯病或系统性红斑狼疮患者的DR和DQ基因限制性片段长度多态性（RFLP）的研究。

Studies of restriction fragment length polymorphism (RFLP) of DR and DQ genes in HLA-DR3 positive patients with Graves' disease or systemic lupus erythematosus.

作者信息

Garcia-Ameijeiras A, Gladman D D, Chan J Y, Volpé R

机构信息

Department of Medicine, Sunnybrook Hospital, University of Toronto, Ontario.

出版信息

Clin Invest Med. 1993 Oct;16(5):326-32.

PMID:7903231

Abstract

We have studied the restriction fragment length polymorphisms (RFLP) of DR and DQ genes in HLA-DR3 positive patients with Graves' disease (GD) or Systemic Lupus Erythematosus (SLE) and compared them to those obtained in DR3 positive normal controls (N). A total of 28 GD patients, 22 SLE, and 24 N were studied. Genomic DNA was digested with BamHI or TaqI restriction enzymes and hybridized with DRB, DQB, or DQA probes. Haplotype distribution between the groups was comparable. No significant differences were found between the fragments obtained in GD and SLE compared to N. In conclusion, there were no specific DR3 polymorphisms detected by RFLP under these conditions in either of the 2 autoimmune disorders.

摘要

我们研究了Graves病（GD）或系统性红斑狼疮（SLE）的HLA - DR3阳性患者中DR和DQ基因的限制性片段长度多态性（RFLP），并将其与DR3阳性正常对照（N）的结果进行比较。共研究了28例GD患者、22例SLE患者和24例正常对照。基因组DNA用BamHI或TaqI限制性内切酶消化，并用DRB、DQB或DQA探针杂交。各组间单倍型分布具有可比性。与正常对照相比，GD和SLE患者获得的片段之间未发现显著差异。总之，在这些条件下，通过RFLP在这两种自身免疫性疾病中均未检测到特定的DR3多态性。