Rospert S, Junne T, Glick B S, Schatz G
Biozentrum, University of Basel, Switzerland.
FEBS Lett. 1993 Dec 13;335(3):358-60. doi: 10.1016/0014-5793(93)80419-u.
The mitochondrial chaperonin system consists of chaperonin 60 (also termed hsp60), which is homologous to E. coli groEL, and chaperonin 10, which is homologous to E. coli groES. In yeast, chaperonin 60 function has been shown to be essential for viability. We report here that the same is true for chaperonin 10. We have cloned, sequenced and disrupted the nuclear chaperonin 10 gene CPN10 from Saccharomyces cerevisiae. This gene encodes a protein of 11,372 Da that is imported into the mitochondrial matrix without detectable cleavage. Haploid cells lacking a functional copy of CPN10 fail to grow at temperatures between 23 and 37 degrees C.
线粒体伴侣蛋白系统由与大肠杆菌groEL同源的伴侣蛋白60（也称为hsp60）和与大肠杆菌groES同源的伴侣蛋白10组成。在酵母中，已证明伴侣蛋白60的功能对于细胞存活至关重要。我们在此报告，伴侣蛋白10也是如此。我们已经克隆、测序并破坏了酿酒酵母的核伴侣蛋白10基因CPN10。该基因编码一种11372道尔顿的蛋白质，该蛋白质可导入线粒体基质而无明显切割。缺乏CPN10功能拷贝的单倍体细胞在23至37摄氏度之间无法生长。
