Shashidharan P, Huntley G W, Meyer T, Morrison J H, Plaitakis A
Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029.
Brain Res. 1994 Oct 31;662(1-2):245-50. doi: 10.1016/0006-8993(94)90819-2.
A cDNA encoding a neuron-specific glutamate/aspartate transporter was isolated from human brain cDNA libraries and characterized. The new cDNA, designated human glutamate transporter III, is structurally distinct from two previously described brain specific glutamate transporters. This human cDNA is 90% and 95% homologous at nucleotide and amino acid level, respectively, with a previously reported rabbit glutamate/aspartate transporter. Northern blot analysis of human tissues revealed that the mRNA of this transporter is expressed in brain, liver, muscle, ovary, testis and in retinoblastoma cell lines. In situ hybridization in human brain sections showed that the mRNA is densely expressed in substantia nigra, red nucleus, hippocampus, and in cerebral cortical layers. Southern blot analysis revealed that the gene encoding this mRNA exists as a single copy in the human genome.
从人脑cDNA文库中分离并鉴定了一个编码神经元特异性谷氨酸/天冬氨酸转运体的cDNA。这个新的cDNA被命名为人类谷氨酸转运体III，在结构上与之前描述的两种脑特异性谷氨酸转运体不同。该人类cDNA在核苷酸和氨基酸水平上分别与先前报道的兔谷氨酸/天冬氨酸转运体有90%和95%的同源性。对人体组织的Northern印迹分析表明，该转运体的mRNA在脑、肝、肌肉、卵巢、睾丸以及视网膜母细胞瘤细胞系中表达。人脑切片的原位杂交显示，该mRNA在黑质、红核、海马以及大脑皮层各层中密集表达。Southern印迹分析表明，编码该mRNA的基因在人类基因组中以单拷贝形式存在。
