Dittadi R, Catozzi L, Gion M, Brazzale A, Capitanio G, Gelli M C, Menegon A, Gardini G, Malagutti R, Piffanelli A
Center for the Study of Biological Markers of Malignancy, Regional General Hospital, Venice, Italy.
Anticancer Res. 1993 Sep-Oct;13(5C):1821-4.
The protein encoded by erbB2 oncogene was evaluated by three different methodological approaches (Western blotting, ELISA and immunohistochemistry) in 147 breast cancer specimens. A highly significant correlation was found between Western blotting and ELISA results (p < 0.001). The data from ELISA and Western blotting were categorized as negatives (-), low-overexpressing (+) and high-overexpressing (++). Immunohistochemical results were classified as (-) or (+) according to the absence or the presence of specific staining. Overall positive cases (+ and ++) were 42.2% by Western blotting and 51% by ELISA, while (++) cases were 23.8% and 25.2% respectively. Histochemical staining was found in 29.8% of cases. The two by two evaluation of the assays showed a close association (chi2, p < 0.0001). In particular, the comparison between both ELISA and Western blotting with immunohistochemistry showed concordance rates of 78.9% for ELISA and 83.1% for Western blotting considering the + and ++ cases as a single group. When only the ++ cases were considered as positive, the overall agreement rises to 93.3% and 89.1% respectively. From these preliminary data we conclude that p185 values obtained with the three evaluated methods are possibly superimposable. Nevertheless, the biochemical methods seem to identify an intermediate p185 expression group, whose clinical meaning should be investigated.
采用三种不同的方法（蛋白质印迹法、酶联免疫吸附测定法和免疫组织化学法）对147例乳腺癌标本中的erbB2癌基因编码蛋白进行了评估。蛋白质印迹法和酶联免疫吸附测定法的结果之间存在高度显著相关性（p<0.001）。酶联免疫吸附测定法和蛋白质印迹法的数据分为阴性（-）、低表达（+）和高表达（++）。免疫组织化学结果根据是否存在特异性染色分为（-）或（+）。蛋白质印迹法检测的总体阳性病例（+和++）为42.2%，酶联免疫吸附测定法为51%，而（++）病例分别为23.8%和25.2%。29.8%的病例发现有组织化学染色。对这些检测方法进行两两评估显示密切相关（卡方检验，p<0.0001）。特别是，将酶联免疫吸附测定法和蛋白质印迹法与免疫组织化学法进行比较时，将+和++病例视为一组，酶联免疫吸附测定法的一致性率为78.9%，蛋白质印迹法为83.1%。当仅将++病例视为阳性时，总体一致性分别升至93.3%和89.1%。从这些初步数据我们得出结论，用三种评估方法获得的p185值可能是重叠的。然而，生化方法似乎识别出一个中间p185表达组，其临床意义有待研究。
