Ong W Y, Garey L J, Reynolds R
Department of Anatomy, National University of Singapore.
J Neurocytol. 1993 Oct;22(10):893-902. doi: 10.1007/BF01186359.
Human cerebral cortex was studied immunocytochemically by light and electron microscopy using antibodies against glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). Glial fibrillary acidic protein-positive cells and processes were present in both cortex and white matter, but in contrast glutamine synthetase-positive cells and processes were present only in cortex. Cell bodies which contained glutamine synthetase had typical ultrastructural features of protoplasmic astrocytes. Glutamine synthetase-positive processes were often present near asymmetrical synapses in the neuropil. These processes often contained mitochondria, but not glial filaments, and were different from unlabelled astrocytic processes, which seldom contained mitochondria, but had large numbers of glial filaments. Glutamine synthetase immunoreactivity therefore affords a means of distinguishing between these two types of astrocytic processes in the human cerebral cortex.
利用抗胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）的抗体，通过光学显微镜和电子显微镜对人类大脑皮层进行了免疫细胞化学研究。胶质纤维酸性蛋白阳性细胞和突起存在于皮层和白质中，但与之形成对比的是，谷氨酰胺合成酶阳性细胞和突起仅存在于皮层中。含有谷氨酰胺合成酶的细胞体具有原浆性星形胶质细胞典型的超微结构特征。谷氨酰胺合成酶阳性突起常常出现在神经毡中的不对称突触附近。这些突起通常含有线粒体，但不含胶质丝，与未标记的星形胶质细胞突起不同，后者很少含有线粒体，但有大量胶质丝。因此，谷氨酰胺合成酶免疫反应性提供了一种区分人类大脑皮层中这两种星形胶质细胞突起的方法。
