Bufler J, Opitz T, Hatt H
Neurologische Klinik, Technischen Universität München, FRG.
Neurosci Lett. 1993 Oct 29;161(2):129-32. doi: 10.1016/0304-3940(93)90276-q.
Granule cells were investigated by performing whole-cell patch-clamp recordings from thin slices of the olfactory bulb of newborn rabbits. Granule cells were intracellularly stained with Lucifer Yellow in their intact environment. During current-clamp measurements these neurones were characterized by their lack of action potentials upon depolarization. Evidence for a Ca2+ dependent K+ conductance was found. Two types of outward currents were identified in the whole cell mode during voltage clamp; a non-inactivating K+ current that shared some properties of the delayed rectifier K+ current and a non-inactivating K+ current were recorded. No fast inward current was registrated.
通过对新生兔嗅球薄片进行全细胞膜片钳记录来研究颗粒细胞。在完整环境中，用荧光黄对颗粒细胞进行细胞内染色。在电流钳测量期间，这些神经元的特征是去极化时缺乏动作电位。发现了一种钙依赖性钾电导的证据。在电压钳的全细胞模式下识别出两种外向电流；记录到一种具有延迟整流钾电流某些特性的非失活钾电流和一种非失活钾电流。未记录到快速内向电流。
