常规免疫组织化学中使用哪些增殖标志物？五种抗体的比较。
Which proliferation markers for routine immunohistology? A comparison of five antibodies.
作者信息
Rose D S, Maddox P H, Brown D C
机构信息
Department of Histopathology, Whittington Hospital, London.
出版信息
J Clin Pathol. 1994 Nov;47(11):1010-4. doi: 10.1136/jcp.47.11.1010.
Abstract
AIMS
To determine the best of five antibodies for immunohistochemical assessment of growth fraction in formalin fixed, paraffin wax embedded tissues.
METHODS
Sections from 100 recent, and 17 ten year old or over wax embedded blocks of normal and malignant tissues were immunostained with monoclonal Ki67, polyclonal Ki67, PC10, MIB1, and JC1. The antibodies were evaluated for specificity of nuclear versus cytoplasmic staining, cleanliness of background, and compared with the expected pattern of staining in normal tissues, defined immunohistochemically by monoclonal Ki67 antibody in frozen tissues or by tritiated thymidine uptake.
RESULTS
No marker was ideal, but best results were obtained with MIB1 and polyclonal Ki67, followed by JC1, PC10, and monoclonal Ki67.
CONCLUSIONS
For routine use, MIB1 or polyclonal Ki67 are the best proliferation markers in conventional histological preparations. The other markers tested cannot be recommended.
摘要
目的
确定用于福尔马林固定、石蜡包埋组织中生长分数免疫组化评估的五种抗体中最佳的一种。
方法
用单克隆Ki67、多克隆Ki67、PC10、MIB1和JC1对来自100个近期以及17个10年及以上石蜡包埋的正常和恶性组织块的切片进行免疫染色。评估这些抗体在细胞核与细胞质染色的特异性、背景的干净程度，并与正常组织中预期的染色模式进行比较，正常组织中的预期染色模式通过冷冻组织中的单克隆Ki67抗体免疫组化定义或通过氚标记胸腺嘧啶核苷摄取来定义。
结果
没有一种标记物是理想的，但MIB1和多克隆Ki67获得了最佳结果，其次是JC1、PC10和单克隆Ki67。
结论
对于常规使用，MIB1或多克隆Ki67是传统组织学制剂中最佳的增殖标记物。不推荐使用所测试的其他标记物。
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