Karita M, Yoshimatsu T, Okita K, Ouchi K
1st Department of Internal Medicine, Yamaguchi University School of Medicine.
Nihon Rinsho. 1993 Dec;51(12):3102-8.
With the goal of differentiating H. pylori in order to clarify its pathogenicity, we have conducted a restriction endonuclease of the chromosomal DNA of 24 strains of H. pylori employing the restriction endonucleases, NotI, CPOI, KpnI, and SmaI using pulsed-field gel electrophoresis (PFGE). With NotI, these 16 strains out of 24 strains were completely digested, and 16 clearly different electrophoretic patterns of NotI digested chromosomal DNA fragments were seen, permitting us to successfully differentiate H. Pylori. We also estimated the genome size of each strain, ranging from 1326 to 2360 kilobases. Using this method, we confirmed our previously established nude mouse model of infection with H. pylori by demonstrating that the mice were infected with the H. pylori administered in this study. Thus, we have demonstrated the digested with NotI by PFGE is an effective method for differentiating H. pylori and estimating the size of DNA of H. pylori.
为了鉴别幽门螺杆菌以阐明其致病性，我们使用NotI、CPOI、KpnI和SmaI等限制性内切酶，通过脉冲场凝胶电泳（PFGE）对24株幽门螺杆菌的染色体DNA进行了限制性内切酶分析。使用NotI时，24株中的16株被完全消化，观察到16种明显不同的NotI消化染色体DNA片段的电泳图谱，这使我们能够成功鉴别幽门螺杆菌。我们还估计了每个菌株的基因组大小，范围为1326至2360千碱基。使用这种方法，我们通过证明本研究中给予小鼠的幽门螺杆菌感染了小鼠，证实了我们先前建立的幽门螺杆菌裸鼠感染模型。因此，我们证明了PFGE用NotI消化是鉴别幽门螺杆菌和估计幽门螺杆菌DNA大小的有效方法。
