Kuo C H, Poon S K, Su Y C, Su R, Chang C S, Wang W C
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
J Infect Dis. 1999 Dec;180(6):2064-8. doi: 10.1086/315130.
Helicobacter pylori strain diversity was investigated in 55 H. pylori seropositive couples in Taiwan in biopsy samples from the antrum and corpus. Two DNA typing methods were used to characterize 90 isolates from 25 couples. In only 1 of the 25 couples was the same strain colonized from both partners. Comparison of isolates from 2 sites in each of 40 patients showed that 9 pairs were distinct but might be related. Peptic ulcer occurred in 77.8% of these 9 patients compared with 29% of 31 patients with the same predominant strain in 2 biopsies (P=.025). Random amplified polymorphic DNA and sequence analyses of 2 closely related isolates from 1 patient support the hypothesis that development of genetic diversity of H. pylori results from horizontal genetic exchange during long-term colonization of mixed bacterial populations.
在台湾，对55对幽门螺杆菌血清学阳性夫妇的胃窦和胃体活检样本中的幽门螺杆菌菌株多样性进行了研究。使用两种DNA分型方法对来自25对夫妇的90株分离株进行了特征分析。在这25对夫妇中，只有1对夫妇的双方定植的是同一菌株。对40名患者中每例患者的两个部位的分离株进行比较，结果显示有9对分离株不同但可能相关。这9例患者中77.8%发生消化性溃疡，而在31例两次活检中主要菌株相同的患者中，这一比例为29%（P = 0.025）。对1例患者的2株密切相关分离株进行随机扩增多态性DNA分析和序列分析，支持了以下假说：幽门螺杆菌遗传多样性的产生是由于在混合细菌群体的长期定植过程中发生水平基因交换。
