Emilson A, Lindberg M, Forslind B, Scheynius A
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Acta Derm Venereol. 1993 Oct;73(5):323-9. doi: 10.2340/0001555573323329.
Quantitative and detailed 3-dimensional (3-D) morphological information can be obtained from the same tissue volume using a confocal laser scanning microscope (CLSM). In the present study, we used CLSM for evaluation of Langerhans' cells (LC) in human skin at 0 h, 24 h and 48 h following occlusion with patch tests for 48 h. The relative volume of epidermal CD1a reactivity was quantified with CLSM on 25 microns thick sections stained with indirect immunofluorescence. No statistically significant difference was found when comparing the values obtained on the biopsy specimens from occluded skin (n = 36) with those from non-occluded skin (n = 9). Nor were any statistically significant changes detected in the number of epidermal CD1a+ cells as determined with immunoperoxidase staining between occluded and non-occluded skin. The occlusion produced a transient mild inflammatory reaction with an induced expression of intercellular adhesion molecule-1 (ICAM-1) on keratinocytes and an increased number of CD3+ epidermal lymphocytes. In addition, 3-D reconstructions revealed spatial information on the distribution of LC dendrites towards the skin surface.
使用共聚焦激光扫描显微镜（CLSM）可以从同一组织体积中获取定量且详细的三维（3-D）形态学信息。在本研究中，我们使用CLSM评估在进行48小时斑贴试验封闭后0小时、24小时和48小时时人皮肤中的朗格汉斯细胞（LC）。在用间接免疫荧光染色的25微米厚切片上，用CLSM对表皮CD1a反应性的相对体积进行定量。将来自封闭皮肤的活检标本（n = 36）与来自未封闭皮肤的活检标本（n = 9）所获得的值进行比较时，未发现统计学上的显著差异。在用免疫过氧化物酶染色确定的封闭皮肤和未封闭皮肤之间，表皮CD1a +细胞的数量也未检测到任何统计学上的显著变化。封闭引起了短暂的轻度炎症反应，角质形成细胞上诱导表达细胞间粘附分子-1（ICAM-1），并且表皮CD3 +淋巴细胞数量增加。此外，三维重建揭示了LC树突向皮肤表面分布的空间信息。
