Ito H, Takekoshi T, Miyauchi M, Ogawa I, Takata T, Nikai H, Takemoto K
Department of Oral Pathology, Hiroshima University School of Dentistry, Japan.
Arch Oral Biol. 1998 Sep;43(9):741-4. doi: 10.1016/s0003-9969(98)00066-1.
Detailed three-dimensional morphological information of the cell without distortion due to thin sectioning can be obtained from thick sections using a confocal laser scanning microscope. Here that microscope was used to evaluate human gingival Langerhans cells stained with anti-CD1a antibody. Optical sectioning and reconstruction by laser scanning microscopy revealed not only three-dimensional aspects of Langerhans cells but also spatial information on the distribution of their dendritic processes towards the gingival surface. The orientated configuration of those processes may reflect the reaction of the gingival Langerhans cell to the surrounding moist environment containing antigenic stimuli originating in saliva.
使用共聚焦激光扫描显微镜，可以从厚切片中获得细胞的详细三维形态信息，而不会因薄切片而产生失真。在此，该显微镜用于评估用抗CD1a抗体染色的人牙龈朗格汉斯细胞。激光扫描显微镜的光学切片和重建不仅揭示了朗格汉斯细胞的三维特征，还显示了其树突状突起向牙龈表面分布的空间信息。这些突起的定向结构可能反映了牙龈朗格汉斯细胞对周围含有源自唾液的抗原刺激的潮湿环境的反应。
