来自患或未患艾滋病儿童的HIV-1分离株具有相似的体外生物学特性。
HIV-1 isolates from children with or without AIDS have similar in vitro biologic properties.
作者信息
Gupta P, Urbach A, Cosentino L, Thampatty P, Brady M
机构信息
Department of Infectious Diseases and Microbiology, University of Pittsburgh, PA 15261.
出版信息
AIDS. 1993 Dec;7(12):1561-4. doi: 10.1097/00002030-199312000-00003.
OBJECTIVE
To compare biologic properties of HIV-1 isolates from children with and without AIDS as a measure of viral cytopathogenicity.
PATIENTS AND PARTICIPANTS
Virus isolates from peripheral blood mononuclear cells of 13 perinatally infected children were compared for specific in vitro biologic properties.
METHODS
Virus isolates were examined for biologic properties as measured by their ability to infect H9 cells and to induce syncytia in susceptible cells.
RESULTS
Most of the pediatric HIV-1 isolates failed to infect CD4+ H9 cells and induce syncytia in susceptible cells, regardless of whether they were from children with or without AIDS. All of the isolates, however, grew well in mitogen-stimulated normal adult lymphocytes. These results are in contrast to those with HIV-1 isolates from adults, whose biologic properties were related to the stages of the disease.
CONCLUSIONS
These results indicate that, unlike adult HIV-1 isolates, the biologic properties of pediatric isolates are not related to the stages of the disease. The rapid development of disease in children may therefore be due to factors other than intrinsic properties of HIV-1 strains present in children.
目的
比较来自患艾滋病和未患艾滋病儿童的人类免疫缺陷病毒1型（HIV-1）分离株的生物学特性，以此作为病毒细胞致病性的一项指标。
患者和参与者
比较了13名围产期感染儿童外周血单个核细胞中的病毒分离株的特定体外生物学特性。
方法
通过检测病毒分离株感染H9细胞以及在易感细胞中诱导合胞体形成的能力来检查其生物学特性。
结果
大多数儿科HIV-1分离株无法感染CD4+H9细胞，也无法在易感细胞中诱导合胞体形成，无论这些分离株来自患艾滋病还是未患艾滋病的儿童。然而，所有分离株在有丝分裂原刺激的正常成人淋巴细胞中生长良好。这些结果与来自成人的HIV-1分离株的结果形成对比，成人分离株的生物学特性与疾病阶段相关。
结论
这些结果表明，与成人HIV-1分离株不同，儿科分离株的生物学特性与疾病阶段无关。因此，儿童疾病的快速发展可能是由于儿童体内存在的HIV-1毒株的内在特性以外的因素所致。
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