Balachandran R, Thampatty P, Enrico A, Rinaldo C, Gupta P
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
Virology. 1991 Jan;180(1):229-38. doi: 10.1016/0042-6822(91)90027-9.
Human immunodeficiency virus type 1 (HIV-1) isolates from asymptomatic homosexual men and AIDS patients were compared for their in vitro biologic and genetic properties. Most of the HIV-1 isolates from asymptomatic men, but not from AIDS patients, failed to infect CD4+ H9 cells and phytohemagglutinin-stimulated peripheral blood lymphocytes. In a longitudinal study, serial HIV-1 isolates obtained from men who seroconverted to HIV-1 and later developed AIDS were able to infect H9 cells. In contrast, longitudinal isolates from men who remained asymptomatic did not infect H9 cells. HIV-1 isolates from AIDS patients in general exhibited increased production of intracellular viral DNA, RNA, and protein as compared to isolates from asymptomatic men. Cells infected with HIV-1 isolates from asymptomatic men produced very little gp120, p24, and p55 proteins as compared to those from AIDS patients. The overall restriction patterns of HindIII, Sac-1, Pst-1, EcoR1, and BamH1 were very similar between HIV-1 isolates from asymptomatic men and those from AIDS patients. However, the restriction endonuclease pattern of BglII was quite distinct for isolates from asymptomatic men as compared to AIDS patients. Preliminary studies mapped a unique BglII site in the gag region of most of the isolates from asymptomatic men, approximately 2.0 kb from the 5' end. Thus, HIV-1 isolates from asymptomatic subjects and from AIDS patients have distinct biologic and genetic properties which may be related to the various clinical outcomes of HIV-1 infection.
对来自无症状同性恋男性和艾滋病患者的1型人类免疫缺陷病毒（HIV-1）分离株的体外生物学和遗传学特性进行了比较。大多数来自无症状男性而非艾滋病患者的HIV-1分离株无法感染CD4 + H9细胞和植物血凝素刺激的外周血淋巴细胞。在一项纵向研究中，从血清转化为HIV-1并随后发展为艾滋病的男性中获得的系列HIV-1分离株能够感染H9细胞。相比之下，来自无症状男性的纵向分离株未感染H9细胞。与来自无症状男性的分离株相比，来自艾滋病患者的HIV-1分离株通常表现出细胞内病毒DNA、RNA和蛋白质产量增加。与来自艾滋病患者的细胞相比，感染来自无症状男性的HIV-1分离株的细胞产生的gp120、p24和p55蛋白非常少。来自无症状男性的HIV-1分离株与来自艾滋病患者的HIV-1分离株之间，HindIII、Sac-1、Pst-1、EcoR1和BamH1的总体限制性图谱非常相似。然而，与艾滋病患者相比，BglII的限制性内切酶图谱在来自无症状男性的分离株中相当不同。初步研究在大多数来自无症状男性的分离株的gag区域中定位了一个独特的BglII位点，距5'端约2.0 kb。因此，来自无症状受试者和艾滋病患者的HIV-1分离株具有不同的生物学和遗传学特性，这可能与HIV-1感染的各种临床结果有关。
