Szymusiak R, McGinty D, Fairchild M D, Jenden D J
Sleep Physiology Laboratory (151A3), Department of Veterans Affairs Medical Center, Sepulveda, CA 91343.
Brain Res. 1993 Nov 26;629(1):141-5. doi: 10.1016/0006-8993(93)90492-6.
Rats reared on a diet in which choline is replaced with N-aminodeanol (NADE), undergo > 50% replacement of brain acetylcholine with acetylated NADE, a false cholinergic transmitter. We examined amounts of sleep and wakefulness in 7 littermate pairs of rats fed either NADE-substituted, or a choline control diet for > 100 days after weaning. During the lights-on portion of the 12/12 h light/dark cycle, NADE rats spent more time awake, and less time in both non-REM and REM sleep compared to littermate controls. Average durations of waking episodes were significantly increased in NADE rats. During the 12 h dark period, there were no between-group differences in sleep-waking amounts. Behavioral hyper-responsiveness which interferes with sleep onset, combined with reduced activity in brainstem cholinergic mechanisms involved in REM sleep generation may underlie daytime sleep-waking disturbances in NADE rats.
用N - 氨基乙醇（NADE）替代胆碱的饮食饲养的大鼠，其大脑中的乙酰胆碱有超过50%被乙酰化NADE替代，乙酰化NADE是一种假性胆碱能递质。我们研究了7对同窝出生的大鼠，在断奶后给它们喂食NADE替代饮食或胆碱对照饮食超过100天，观察它们的睡眠和觉醒量。在12/12小时明暗循环的光照期，与同窝对照相比，NADE大鼠清醒时间更长，非快速眼动睡眠和快速眼动睡眠的时间更少。NADE大鼠清醒发作的平均时长显著增加。在12小时黑暗期，两组之间的睡眠 - 觉醒量没有差异。干扰睡眠起始的行为高反应性，与参与快速眼动睡眠产生的脑干胆碱能机制活动减少相结合，可能是NADE大鼠白天睡眠 - 觉醒障碍的基础。
