Pettengill O S, Memoli V A, Brinck-Johnsen T, Longnecker D S
Department of Pathology, Dartmouth Medical School, Lebanon, NH 03756.
Carcinogenesis. 1994 Jan;15(1):61-5. doi: 10.1093/carcin/15.1.61.
Continuous cell lines have been isolated from islet cell, small cell anaplastic and acinar cell carcinomas arising in the pancreas of transgenic mice, line Tg(Ela-1-SV40E)Bri18. These mice carry the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen. Cells derived from islet cell or small cell anaplastic tumors secreted insulin and somatostatin during the early period of culture. Phenotypic alterations occurred during culture, whereby insulin secretion ceased and cells instead secreted somatostatin, indicating a change from beta-cell to delta-cell phenotype. Acinar cell lines did not secrete amylase or lipase.
连续细胞系已从转基因小鼠（Tg(Ela-1-SV40E)Bri18品系）胰腺中产生的胰岛细胞、小细胞间变性癌和腺泡细胞癌中分离出来。这些小鼠携带由与SV40 T抗原相连的弹性蛋白酶-1启动子组成的假基因构建体。源自胰岛细胞或小细胞间变性肿瘤的细胞在培养早期分泌胰岛素和生长抑素。培养过程中发生了表型改变，胰岛素分泌停止，细胞转而分泌生长抑素，表明从β细胞表型转变为δ细胞表型。腺泡细胞系不分泌淀粉酶或脂肪酶。
