Jira T, Breyer C
Institut für Arzneimittelkontrolle, Ernst-Moritz-Arndt-Universität Greifswald.
Pharmazie. 1993 Nov;48(11):829-33.
Investigations on direct separation by RP-HPLC of selected enantiomeric beta-adrenergic active agents are described. R- and S-1-phenylethylisocyanate (PEIC) as well as (1S)-(+)-campher-10-sulfonylchloride (CSC) are used for the derivatization of the compounds. Correlations between chromatographic data and various influences (pH, temperature, reaction time, concentration of reagens a.o.) have been discussed. The behaviour of derivatized and underivatized analytes on RP-columns is described in dependence on buffer concentration of the mobile phase. The investigated beta-adrenergic agents were partly separated after derivatization with PEIC, whereas this was not possible with CSC as derivatization reagent.
描述了关于通过反相高效液相色谱法（RP-HPLC）直接分离选定的对映体β-肾上腺素能活性剂的研究。使用R-和S-1-苯基异氰酸酯（PEIC）以及（1S）-（+）-樟脑-10-磺酰氯（CSC）对化合物进行衍生化。讨论了色谱数据与各种影响因素（pH、温度、反应时间、试剂浓度等）之间的相关性。根据流动相的缓冲液浓度，描述了衍生化和未衍生化分析物在反相柱上的行为。在用PEIC衍生化后，所研究的β-肾上腺素能剂部分得以分离，而使用CSC作为衍生化试剂则无法实现分离。
