Bourinbaiar A S, Tan X, Nagorny R
Department of Pediatrics, NYU Medical Center, NY 10016.
Acta Virol. 1993 Aug;37(4):241-50.
A single dose of coumarin derivatives, warfarin, 4-hydroxycoumarin and umbelliferone, added at the time of inoculation either by free virus or by contact with U1 monocytes exhibited a dose-dependent inhibitory effect on viral replication in target MOLT-4 lymphocytes observable even at 5 days post infection. In addition, marked decrease of HIV-1 gap p24 release and reduction in reverse transcriptase activity was observed when chronically HIV-infected ACH-2 lymphocytes were treated with coumarins (ED50% range 10(-6)-10(-9) mol/l). However, the intracellular composition of HIV-1 core proteins in drug-exposed cells was not modified. Results suggest that although no complete inhibition of viral production has been observed in vitro this class of drugs may present potential interest as antiviral agents.
在接种时添加单剂量的香豆素衍生物、华法林、4-羟基香豆素和伞形酮，无论是通过游离病毒还是通过与U1单核细胞接触，即使在感染后5天也可观察到对靶MOLT-4淋巴细胞中病毒复制的剂量依赖性抑制作用。此外，当用香豆素处理慢性HIV感染的ACH-2淋巴细胞时，观察到HIV-1缺口p24释放明显减少且逆转录酶活性降低（ED50%范围为10(-6)-10(-9)mol/L）。然而，药物处理细胞中HIV-1核心蛋白的细胞内组成未发生改变。结果表明，尽管在体外未观察到对病毒产生的完全抑制，但这类药物可能作为抗病毒剂具有潜在的应用价值。
