在大肠杆菌中鉴定出第二种聚腺苷酸聚合酶。
Identification of a second poly(A) polymerase in Escherichia coli.
作者信息
Kalapos M P, Cao G J, Kushner S R, Sarkar N
机构信息
Boston Biomedical Research Institute, MA 02114.
出版信息
Biochem Biophys Res Commun. 1994 Jan 28;198(2):459-65. doi: 10.1006/bbrc.1994.1067.
A second poly(A) polymerase (PAP II) has been identified in Escherichia coli using a strain carrying a deletion of pcnB (the structural gene for PAP I; Cao and Sarkar, 1992b) and pnp-7 (a null mutation in the structural gene for polynucleotide phosphorylase). While PAP I has a M(r) of 53,000, PAP II is a smaller protein with a native M(r)-35,000. PAP II differs from PAP I in preferring poly(A) over tRNA primers and being more thermolabile. The presence of multiple poly(A) polymerases in E. coli raises interesting questions regarding the role of polyadenylation in mRNA synthesis and decay.
利用携带pcnB（聚腺苷酸聚合酶I的结构基因；曹和萨卡尔，1992b）缺失和pnp - 7（多核苷酸磷酸化酶结构基因中的无效突变）的大肠杆菌菌株，已在大肠杆菌中鉴定出第二种聚腺苷酸聚合酶（聚腺苷酸聚合酶II）。聚腺苷酸聚合酶I的相对分子质量为53,000，而聚腺苷酸聚合酶II是一种较小的蛋白质，其天然相对分子质量为35,000。聚腺苷酸聚合酶II与聚腺苷酸聚合酶I的不同之处在于，它更倾向于以聚腺苷酸而非tRNA作为引物，并且对热更不稳定。大肠杆菌中存在多种聚腺苷酸聚合酶，这就聚腺苷酸化在mRNA合成和降解中的作用提出了有趣的问题。
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