Haugel-Nielsen J, Hajnsdorf E, Regnier P
Institut de Biologie Physico-Chimique, Paris, France.
EMBO J. 1996 Jun 17;15(12):3144-52.
The rpsO monocistronic messenger, encoding ribosomal protein S15, is destabilized upon polyadenylation occurring at the hairpin structure of the transcription terminator t1. We report that mRNA fragments differing from the monocistronic transcript by their 3' termini are also polyadenylated in the absence of polynucleotide phosphorylase and RNase II. Some of these 3' extremities result from endonucleolytic cleavages by RNase E and RNase III and from exonucleolytic degradation. Most of these mRNA fragments are destabilized upon polyadenylation with the exception of the RNA species generated by RNase III. RNase E appears to reduce the amount of poly(A) added at the transcription terminator t1.
编码核糖体蛋白S15的rpsO单顺反子信使RNA，在转录终止子t1的发夹结构处发生聚腺苷酸化后会变得不稳定。我们报告称，在没有多核苷酸磷酸化酶和核糖核酸酶II的情况下，其3'末端与单顺反子转录本不同的mRNA片段也会发生聚腺苷酸化。其中一些3'末端是由核糖核酸酶E和核糖核酸酶III的内切核酸酶切割以及外切核酸酶降解产生的。除了由核糖核酸酶III产生的RNA种类外，这些mRNA片段中的大多数在聚腺苷酸化后都会变得不稳定。核糖核酸酶E似乎会减少在转录终止子t1处添加的聚腺苷酸的量。
