Jonsson J
Scand J Rheumatol. 1976;5(3):177-83. doi: 10.3109/03009747609165459.
Sera with an antinuclear immunofluorescence titre of 1/000 were taken consecutively from the diagnostic routine flow and examined for agglutinating antibodies against desoxyribonucleic acid (DNA) and extractable nuclear antigens (ENA). Passive haemagglutination tests with antigen-coated tanned erythrocytes were used and the specificity of the reactions was corroborated by testing against enzyme-treated cells. After the exclusion of the DNA-reacting 15%, three major groups and one minor could be distinguished on a serological basis. The largest group (41%) contained cases with a speckled immunofluorescence pattern and a RNase-trypsin sensitive agglutination reaction with ENA coated cells (sRNP). Nearly all cases of mixed connective tissue disease and scleroderma fell into this group which also contained 44% of the SLE cases. Symptomatically the group was characterized by remarkably high incidences of Raynaud's syndrome and myositis. The major group next in size comprised cases with a homogeneous immunofluorescence pattern but no reaction against DNA or ENA. Half of the cases within this group had the diagnosis SLE; they also constituted 42% of all SLE cases. The only other diagnosis of significant frequency within the group was unspecified collagenosis (23%). The symptomatology of the group was rather uncharacteristic, with the exception of the low incidence of Raynaud's syndrome. The third major group comprised cases with a speckled immunofluorescence pattern but no agglutination reaction against ENA or DNA. This group had a very high incidence of rheumatoid factor and also the highest incidence of visceral lesions among the groups. Yet the group contained only a small proportion (14%) of the SLE cases and the rheumatoid arthritis cases were about equally shared between this and the first group. The most common diagnosis in the group was unspecified collagenosis (40%). A fourth, small but homogeneous group contained cases with a speckled immunofluorescence pattern and a reaction with Sm antigen, i.e. an enzyme-resistant agglutination reaction with ENA. Six cases in this group had the diagnosis SLE. No diagnosis was available in two cases.
从诊断常规流程中连续选取抗核免疫荧光滴度为1/1000的血清，检测其针对脱氧核糖核酸（DNA）和可提取核抗原（ENA）的凝集抗体。采用抗原包被的鞣酸红细胞进行被动血凝试验，并通过对酶处理细胞进行检测来确证反应的特异性。排除与DNA反应的15%后，在血清学基础上可区分出三大组和一小组。最大的一组（41%）包括免疫荧光呈斑点状、对ENA包被细胞（sRNP）有核糖核酸酶 - 胰蛋白酶敏感凝集反应的病例。几乎所有混合性结缔组织病和硬皮病病例都属于这一组，其中还包括44%的系统性红斑狼疮（SLE）病例。从症状上看，该组的特点是雷诺综合征和肌炎的发病率显著较高。第二大组包括免疫荧光呈均质状、但对DNA或ENA无反应的病例。该组一半的病例诊断为SLE；它们也占所有SLE病例的42%。该组中另一个有显著频率的诊断是未明确的胶原病（23%）。除了雷诺综合征发病率较低外，该组的症状相当不典型。第三大组包括免疫荧光呈斑点状、但对ENA或DNA无凝集反应的病例。该组类风湿因子的发病率非常高，在内脏病变方面也是各组中发病率最高的。然而，该组仅包含一小部分（14%）的SLE病例，类风湿关节炎病例在该组和第一组之间大致平均分布。该组最常见的诊断是未明确的胶原病（40%）。第四组规模小但病例均一，包括免疫荧光呈斑点状、与Sm抗原发生反应的病例，即对ENA有酶抗性凝集反应的病例。该组有6例诊断为SLE。2例未明确诊断。
