Pierrefiche G, Zerbib R, Laborit H
Laboratoire d'Eutonologie-Hopital Boucicaut, Paris, France.
Res Commun Chem Pathol Pharmacol. 1993 Nov;82(2):131-42.
The anxiolytic properties of melatonin are revealed by two behavioral studies. In a free exploratory situation, the holeboard test, melatonin decreased head-dip performance. In an unconditioned conflict test, the light/dark box choice situation, melatonin increased the time spent in the lit box as well as the number of transitions between the two compartments. Melatonin was given in a dose range from 0.5 to 5.0 mg/kg body weight i.p. 30 minutes before testing in daytime. Moreover, the anxiolytic activity of diazepam (2.5 mg/kg i.p.) was evaluated and found to be completely inhibited by the specific benzodiazepine antagonist flumazenil (10 mg/kg i.p. 30 minutes before). In the same manner flumazenil counteracted melatonin activity in the two tests. Involvement of the benzodiazepine/GABAergic system in the anxiolytic activity of melatonin is discussed.
两项行为学研究揭示了褪黑素的抗焦虑特性。在自由探索情境即洞板试验中，褪黑素降低了探洞行为表现。在非条件冲突试验即明暗箱选择情境中，褪黑素增加了在亮箱中停留的时间以及在两个隔室之间转换的次数。在白天测试前30分钟，以0.5至5.0毫克/千克体重的剂量腹腔注射给予褪黑素。此外，评估了地西泮（2.5毫克/千克腹腔注射）的抗焦虑活性，发现其被特异性苯二氮䓬拮抗剂氟马西尼（10毫克/千克腹腔注射，提前30分钟）完全抑制。同样，氟马西尼在两项试验中抵消了褪黑素的活性。文中讨论了苯二氮䓬/γ-氨基丁酸能系统在褪黑素抗焦虑活性中的作用。
