Niehrs C, Steinbeisser H, De Robertis E M
Molecular Biology Institute, University of California, Los Angeles 90024-1737.
Science. 1994 Feb 11;263(5148):817-20. doi: 10.1126/science.7905664.
Amphibian mesoderm arises from the marginal zone of the early gastrula and generates various tissues such as notochord, muscle, kidney, and blood. Small changes (twofold) in the amount of microinjected messenger RNA encoding the goosecoid (gsc) homeodomain protein resulted in marked changes in the differentiation of mesoderm in Xenopus laevis. At least three thresholds were observed, which were sufficient to specify four mesodermal cell states. Endogenous gsc messenger RNA was expressed in the marginal zone in a graded fashion that is congruent with a role for this gene in dorso-ventral patterning of mesoderm at the early gastrula stage.
两栖动物的中胚层起源于早期原肠胚的边缘区，并产生各种组织，如脊索、肌肉、肾脏和血液。微量注射编码鹅膏草碱（gsc）同源域蛋白的信使核糖核酸数量上的微小变化（两倍），导致非洲爪蟾中胚层分化发生显著变化。至少观察到三个阈值，这些阈值足以确定四种中胚层细胞状态。内源性gsc信使核糖核酸以梯度方式在边缘区表达，这与该基因在早期原肠胚阶段中胚层背腹模式形成中的作用一致。
