Niehrs C, Keller R, Cho K W, De Robertis E M
Molecular Biology Institute, University of California, Los Angeles 90024-1737.
Cell. 1993 Feb 26;72(4):491-503. doi: 10.1016/0092-8674(93)90069-3.
Goosecoid (gsc), a homeobox gene expressed specifically in the dorsal blastopore lip of the Xenopus gastrula, is considered to play an important role in Spemann's organizer phenomenon. Lineage tracing and time-lapse microscopy were used to follow the fate of embryonic cells microinjected with gsc mRNA. Microinjected gsc has non-cell autonomous effects, recruiting neighboring uninjected cells into a twinned dorsal axis. Ectopic expression of gsc mRNA in ventral blastomeres as well as overexpression of gsc in dorsal blastomeres leads to cell movement toward the anterior of the embryo. The results suggest a function for gsc in the control of gastrulation movements in groups of cells, but not in dissociated cells, and demonstrate that a vertebrate homeobox gene can regulate region-specific cell migration.
鹅膏蕈氨酸同源物（gsc）是一种在非洲爪蟾原肠胚的背侧胚孔唇中特异性表达的同源异型盒基因，被认为在施佩曼组织者现象中发挥重要作用。采用谱系追踪和延时显微镜技术来追踪显微注射gsc mRNA的胚胎细胞的命运。显微注射的gsc具有非细胞自主效应，可将相邻未注射的细胞募集到孪生背轴中。gsc mRNA在腹侧卵裂球中的异位表达以及gsc在背侧卵裂球中的过表达都会导致细胞向胚胎前部移动。结果表明，gsc在控制细胞群的原肠胚形成运动中发挥作用，但在解离的细胞中不起作用，并且证明脊椎动物的同源异型盒基因可以调节区域特异性细胞迁移。
