Charaf B, Sanhadji K, Sekkat S, Farouqui B, Touraine J L, Benslimane A
Laboratoire d'Immunovirologie, Institut Pasteur du Maroc, Casablanca.
Thymus. 1993 Aug;22(1):1-12.
The anti-retrovirus cell-mediated immunity was repeatedly investigated in seven monkeys (Macaca sylvana). Four of these animals were injected with cell-free supernatants containing human immunodeficiency viruses: two monkeys received HIV1 Bru (2.5 x 10(6) cpm), two received HIV2 Rod (1.5 x 10(6) cpm). Two additional animals were injected with a cell-free supernatant containing simian immunodeficiency virus SIV/mac 251 (1.5 x 10(6) cpm) and the last animal served as control. The four macaques infected with HIV2 Rod and SIV/mac 251 seroconverted. Freshly isolated and non stimulated peripheral blood mononuclear cells from these infected macaques and from the uninfected control were repeatedly assessed for cytolytic activity. Target cells consisted of heterologous human cell lines expressing HIV1 Bru, HIV2 Rod or SIV/mac proteins. A significant cytotoxic activity, non-restricted at the major histocompatibility complex class I (MHC-I), was demonstrated in one HIV2 Rod-infected animal (F8) and in one SIV/mac 251-infected animal (M1). This last animal showed progressively diminishing cytolytic activity that was correlated with a pronounced decrease in CD4+ lymphocytes. An AIDS-like disease developed in M1, with presence of lymphadenopathy, weight loss, diarrhea and opportunistic infections. Cytotoxic activity was active against SIV and HIV2-infected target cells in an MHC-unrestricted manner; it was specific to virus-infected cells and there was cross-reactivity between HIV2 and SIV. Cytotoxic effectors appeared to be mainly CD8+ cells. This model may prove to be very useful in evaluating the capacity of candidate AIDS vaccines to elicit effective cell-mediated immune responses.
对7只地中海猕猴反复进行了抗逆转录病毒细胞介导免疫研究。其中4只动物注射了含人类免疫缺陷病毒的无细胞上清液：2只猴子接受了HIV1 Bru（2.5×10⁶ cpm），2只接受了HIV2 Rod（1.5×10⁶ cpm）。另外2只动物注射了含猴免疫缺陷病毒SIV/mac 251的无细胞上清液（1.5×10⁶ cpm），最后1只动物作为对照。感染HIV2 Rod和SIV/mac 251的4只猕猴血清阳转。对这些感染猕猴和未感染对照的新鲜分离且未受刺激的外周血单核细胞反复进行细胞溶解活性评估。靶细胞由表达HIV1 Bru、HIV2 Rod或SIV/mac蛋白的异源人类细胞系组成。在1只感染HIV2 Rod的动物（F8）和1只感染SIV/mac 251的动物（M1）中显示出显著的细胞毒性活性，该活性不受主要组织相容性复合体I类（MHC-I）限制。最后这只动物的细胞溶解活性逐渐降低，这与CD4⁺淋巴细胞的显著减少相关。M1出现了类似艾滋病的疾病，伴有淋巴结病、体重减轻、腹泻和机会性感染。细胞毒性活性以MHC非限制方式对SIV和HIV2感染的靶细胞有活性；它对病毒感染细胞具有特异性，并且HIV2和SIV之间存在交叉反应性。细胞毒性效应细胞似乎主要是CD8⁺细胞。该模型可能在评估候选艾滋病疫苗引发有效细胞介导免疫反应的能力方面被证明非常有用。
