Gotch F, McAdam S N, Allsopp C E, Gallimore A, Elvin J, Kieny M P, Hill A V, McMichael A J, Whittle H C
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
J Immunol. 1993 Sep 15;151(6):3361-9.
A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.
对9名感染HIV-2的冈比亚患者进行了针对gag特异性、MHC限制性CD8+细胞毒性T淋巴细胞（CTL）的初步研究。在新鲜外周血单个核细胞中，至少55%的患者存在此类CTL，无需体外再刺激。我们利用从HLA-B53分子洗脱的内源性肽段分析预测的氨基酸序列基序，鉴定出一种来自HIV-2 gag的九聚体肽，该肽可被CD8+HLA-B53 CTL识别。这种来自HIV-2/SIV保守序列的肽，此前曾报道可被接种表达SIV gag蛋白的重组痘苗病毒或感染SIV病毒的非人灵长类动物的CTL识别。HLA-B53限制性、HIV-2 gag特异性CTL不识别表达HIV-1 gag蛋白的靶细胞，表明在这种情况下无法预期对HIV-1有细胞交叉保护作用。
