二氟苯脲对新蜕皮的美洲大蠊（Periplaneta americana）经通透处理及分离的体壁中UDP-N-乙酰-[3H]葡糖胺（UDP-[3H]NAGA）掺入几丁质的影响。
Effect of diflubenzuron on the incorporation of UDP-N-acetyl-[3H]glucosamine (UDP-[3H]NAGA) to chitin in permeabilized, and isolated integuments from the newly molted American cockroach Periplaneta americana.
作者信息
Nakagawa Y, Matsumura F
机构信息
Department of Environmental Toxicology, University of California, Davis 95616.
出版信息
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Nov;106(3):705-10. doi: 10.1016/0742-8413(93)90230-i.
PMID:7905807
Abstract
- Diflubenzuron (DFB) was found to inhibit the incorporation of UDP-N-acetyl-[3H]glucosamine (UDP-[3H]NAGA) to chitin in permeabilized and isolated integument from newly molted American cockroach. 2. The favorable experimental conditions demonstrating the effect of diflubenzuron were: 10 mM phosphate, low calcium concentration (10(-6) M-10(-8) M), high potassium concentration (> 100 mM), and high pH (> or = 7). 3. The action of diflubenzuron was completely erased by preincubating the isolated integument with valinomycin, FCCP, or A23187. 4. By lowering the external pH to 5.2, it was also possible to reduce the rate of UDP-[3H]NAGA incorporation to the extent that DFB's effect was no longer recognizable. 5. Both Cs+ and Rb+ could replace K+ in maintaining a high level of chitin synthesis and the inhibitory action of DFB under the optimum conditions.
摘要
- 研究发现，除虫脲（DFB）可抑制新蜕皮的美国蟑螂经通透处理和分离的体壁中UDP-N-乙酰-[3H]葡糖胺（UDP-[3H]NAGA）掺入几丁质。2. 证明除虫脲作用的适宜实验条件为：10 mM磷酸盐、低钙浓度（10⁻⁶ M - 10⁻⁸ M）、高钾浓度（> 100 mM）和高pH值（≥ 7）。3. 用缬氨霉素、羰基氰对三氟甲氧基苯腙（FCCP）或A23187预孵育分离的体壁，可完全消除除虫脲的作用。4. 将外部pH值降至5.2，也可降低UDP-[3H]NAGA掺入速率，使除虫脲的作用不再明显。5. 在最佳条件下，Cs⁺和Rb⁺均可替代K⁺维持高水平的几丁质合成及除虫脲的抑制作用。
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