Chester K A, Begent R H, Robson L, Keep P, Pedley R B, Boden J A, Boxer G, Green A, Winter G, Cochet O
Royal Free Hospital School of Medicine, London, UK.
Lancet. 1994 Feb 19;343(8895):455-6. doi: 10.1016/s0140-6736(94)92695-6.
Insertion of antibody genes into filamentous bacteriophage makes it possible to generate and screen libraries of 10(7) or more antibodies. Each phage expresses an antibody on its surface and contains the corresponding antibody gene. Genes that encode antibodies with desired characteristics are readily selected and their antibodies expressed as soluble proteins in Escherichia coli. We used this system to produce an antibody to carcinoembryonic antigen with higher affinity and better tumour specificity than antibodies currently in use.
将抗体基因插入丝状噬菌体中，使得生成和筛选10⁷或更多抗体的文库成为可能。每个噬菌体在其表面表达一种抗体，并包含相应的抗体基因。编码具有所需特性抗体的基因很容易被筛选出来，并且它们的抗体可以在大肠杆菌中作为可溶性蛋白表达。我们使用这个系统制备了一种针对癌胚抗原的抗体，其亲和力高于目前使用的抗体，且肿瘤特异性更好。
