产半乳糖氧化酶霉菌线粒体DNA的限制性内切酶片段模式

Restriction enzyme fragment patterns of mtDNA from a galactose oxidase-producing mold.

Pereira I M, Zancan G T

Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, Brasil.

Braz J Med Biol Res. 1993 Oct;26(10):1047-55.

Abstract

Mitochondrial DNAs from Dactylium dendroides, Hypomyces rosellus, Fusarium graminearum, Gibberella fujikuroi, Fusarium tricinctum strains and a galactose oxidase (GAO)-producing mold (original strain) presented distinctive restriction enzyme fragment patterns with the endonucleases Hind III and EcoRI. 2. A small number of comigrating bands was found when the GAO-producing mold was compared with the others. The molecular size of mtDNA from the GAO-producing mold, as judged by summation of fragment sizes produced by digestion with EcoRI, Hind III and Bgl II, is 61.3 +/- 2.16 kb. 3. The results suggest that the mtDNA from the GAO-producing mold strain is distinct from that of D. dendroides and all other ascomycetes analyzed.

摘要

来自树状指孢霉、玫瑰色拟层孔菌、禾谷镰刀菌、藤仓赤霉、三线镰刀菌菌株以及一株产半乳糖氧化酶（GAO）的霉菌（原始菌株）的线粒体DNA，在用内切酶Hind III和EcoRI切割时呈现出独特的限制性酶切片段模式。2. 将产GAO的霉菌与其他菌株进行比较时，发现了少量共迁移条带。通过对用EcoRI、Hind III和Bgl II消化产生的片段大小求和判断，产GAO霉菌的线粒体DNA分子大小为61.3±2.16 kb。3. 结果表明，产GAO霉菌菌株的线粒体DNA与树状指孢霉以及所有其他分析过的子囊菌的线粒体DNA不同。