Crickmore N, Wheeler V C, Ellar D J
Department of Biochemistry, University of Cambridge, UK.
Mol Gen Genet. 1994 Feb;242(3):365-8. doi: 10.1007/BF00280428.
A delta-endotoxin gene previously cloned from Bacillus thuringiensis subsp. galleriae has been shown by a combination of restriction mapping and DNA sequence analysis to be a cryIIB clone; in common with other cryIIB genes it was found to lack a functional promoter. Addition of a promoter resulted in expression of the gene in Bacillus thuringiensis but did not result in the formation of the crystalline inclusions normally associated with such toxins. Inclusion formation was only observed when the gene was incorporated into an operon containing a gene known to be involved in the crystallisation of another delta-endotoxin.
先前从苏云金芽孢杆菌蜡螟亚种中克隆出的一种δ-内毒素基因，经限制性酶切图谱分析和DNA序列分析相结合，已证明是一种cryIIB克隆；与其他cryIIB基因一样，发现它缺乏功能性启动子。添加启动子导致该基因在苏云金芽孢杆菌中表达，但并未导致通常与此类毒素相关的晶体包涵体形成。只有当该基因被整合到一个操纵子中时才观察到包涵体形成，该操纵子包含一个已知参与另一种δ-内毒素结晶的基因。
