表达新型杀虫δ-内毒素组合的苏云金芽孢杆菌菌株的构建
The construction of Bacillus thuringiensis strains expressing novel entomocidal delta-endotoxin combinations.
作者信息
Crickmore N, Nicholls C, Earp D J, Hodgman T C, Ellar D J
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1990 Aug 15;270(1):133-6. doi: 10.1042/bj2700133.
Abstract
Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone & Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal delta-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.
摘要
利用我们最近报道的电穿孔法转化苏云金芽孢杆菌[Bone和Ellar（1989年），《FEMS微生物学快报》58卷，第171 - 178页]，已将克隆的苏云金芽孢杆菌昆虫icidalδ-内毒素基因导入几种天然苏云金芽孢杆菌菌株。在许多情况下，所得转化体既表达其天然毒素，又表达克隆毒素，从而产生具有更广泛毒性谱的菌株。将暗黑变种毒素基因导入苏云金芽孢杆菌以色列变种，产生了一种对粉纹夜蛾（菜粉蝶）有活性的菌株，而亲本菌株均不具备这种活性。我们进一步讨论了协同作用的可能性以及通过这种方法引入克隆DNA所涉及的问题。
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