Jadin J, Timperman G, De Ruysser F
Trans R Soc Trop Med Hyg. 1976;70(3):235-7. doi: 10.1016/0035-9203(76)90046-8.
The authors describe the characters in two pyrimethamine-resistant lines of Plasmodium berghei berghei SP 11-RR after long storage at very low temperatures. In one case the line had maintained its original virulence but lost its resistance to pyrimethamine. Gametocytogenesis increased and cyclical transmission was successful. Furthermore, parasites crossed the blood-brain barrier and provoked cerebral malaria. In the other case no physiological changes could be detected. The authors conclude that, after cryopreservation, biological behaviour should always be carefully observed.
作者描述了伯氏疟原虫伯氏亚种SP 11-RR的两个耐乙胺嘧啶品系在极低温度下长期保存后的特性。在一个案例中，该品系保持了其原始毒力，但失去了对乙胺嘧啶的抗性。配子体生成增加，周期性传播成功。此外，寄生虫穿过血脑屏障并引发脑型疟疾。在另一个案例中，未检测到生理变化。作者得出结论，冷冻保存后，应始终仔细观察生物学行为。
