Clarke R A, Beh K J
CSIRO Division of Animal Health, McMaster Laboratory, Glebe, N.S.W., Australia.
Vet Immunol Immunopathol. 1993 Dec;39(4):381-93. doi: 10.1016/0165-2427(93)90069-g.
A cosmid clone containing the ovine and C epsilon and C alpha immunoglobulin heavy chain genes was isolated and characterised. Restriction mapping and sequence analysis showed a high degree of similarity between the bovine and ovine C epsilon loci. Restriction fragment length polymorphism (RFLP) analysis of sheep genomic DNA revealed Mendelian inheritance of polymorphisms with identical variation in allele size for various restriction enzymes. This identical variation suggested that a deletion of approximately 100 bp existed at the 5' end of the smaller alleles.
分离并鉴定了一个包含绵羊Cε和Cα免疫球蛋白重链基因的黏粒克隆。限制性酶切图谱和序列分析表明，牛和绵羊的Cε基因座之间具有高度相似性。对绵羊基因组DNA的限制性片段长度多态性（RFLP）分析显示，多态性符合孟德尔遗传规律，不同限制性内切酶的等位基因大小存在相同的变异。这种相同的变异表明，较小等位基因的5'端存在约100 bp的缺失。
