Desai M, Linton D, Owen R J, Stanley J
National Collection of Type Cultures, Central Public Health Laboratory, London.
Epidemiol Infect. 1994 Feb;112(1):151-60. doi: 10.1017/s0950268800057514.
The gastric-adapted bacterium Helicobacter pylori plays an important role in gastritis and ulcer disease, but no phenotypic typing scheme presently exists for this organism. With a view to the development of genotypic typing, we have compared isolates of H. pylori from gastritis or ulcer patients with those from subjects exhibiting no disease. Variation was analysed at the urease genes, ureA and ureCD, by employing PCR-generated probes in genomic Southern blot hybridizations. Whilst ureA restriction fragments provided a fourfold subgrouping of strains, ureCD fragments were considerably more discriminatory. Twenty-four combined ureACD profiles were generated with Hind III, subdividing the 64 strains into 11 types and 13 single profiles. The most prevalent profile (UI) was found in 33% of strains, almost all from gastritis or ulcer patients. On the other hand strains isolated from asymptomatic individuals had the most diverse ureACD profiles. A key finding from this set of isolates was that strains of H. pylori associated with general gastroduodenal disease were genetically more homogeneous than strains carried by people without disease symptoms.
适应胃内环境的细菌幽门螺杆菌在胃炎和溃疡病中起重要作用，但目前尚无针对该菌的表型分型方案。为了开展基因型分型，我们比较了来自胃炎或溃疡患者的幽门螺杆菌分离株与来自无疾病症状受试者的分离株。通过在基因组Southern印迹杂交中使用PCR产生的探针，分析了脲酶基因ureA和ureCD的变异情况。虽然ureA限制性片段可将菌株分为四个亚组，但ureCD片段的鉴别力更强。用Hind III产生了24种ureACD组合图谱，将64株菌株分为11种类型和13种单图谱。最常见的图谱（UI）在33%的菌株中出现，几乎所有菌株都来自胃炎或溃疡患者。另一方面，从无症状个体分离出的菌株具有最多样化的ureACD图谱。这组分离株的一个关键发现是，与一般胃十二指肠疾病相关的幽门螺杆菌菌株在基因上比无疾病症状者携带的菌株更具同质性。
