Repopulation of tumour cells following irradiation with X-rays or low energy neutrons.

The repopulation of C3H mouse mammary carcinoma cells following X-ray or fast neutron irradiation was investigated in vivo using TCD50 as an endpoint. Tumours in the C3H mouse leg were irradiated in air with an X-ray dose of 9.6, 28.8 or 48.0 Gy, or a neutron dose of 2.6 or 5.2 Gy, and, various times thereafter, graded X-ray doses were given under hypoxic conditions to determine TCD50. Substantial recovery from sublethal and potentially lethal radiation damages was observed within 48 h following X-ray irradiation, and less recovery was found after neutrons. The effective number of tumourigenic cells was calculated at the time of the second irradiation using the TCD50 equation based on the multitarget model. The effective cell doubling time following X-rays depended on radiation dose. Repopulation appeared to be faster after small and intermediate X-ray doses (9.6 and 28.8 Gy) than after the largest dose, but substantial division delay was observed after the largest X-ray dose. A significant finding was that the tumour cells treated with a neutron dose, either 2.6 or 5.2 Gy, appeared to repopulate slightly more rapidly than those irradiated with X-rays, and the doubling times following two different neutron doses were not significantly different. These results suggest the use of a short overall treatment time for the high LET radiotherapy, and that caution must be exercised if the high LET radiation is given before photon doses.