番茄中编码二氢黄酮醇4-还原酶的基因的特性分析
Characterization of the gene encoding dihydroflavonol 4-reductase in tomato.
作者信息
Bongue-Bartelsman M, O'Neill S D, Tong Y, Yoder J I
机构信息
Department of Vegetable Crops, University of California-Davis 95616-8746.
出版信息
Gene. 1994 Jan 28;138(1-2):153-7. doi: 10.1016/0378-1119(94)90799-4.
A cDNA clone (DFR) encoding dihydroflavonol 4-reductase was identified from tomato hypocotyls. Nucleotide and amino acid sequence comparisons to Petunia hybrida, Antirrhinum majus and Zea mays DFR sequences confirmed that the cDNA encodes the structural DFR gene. In tomato, the DFR sequence appeared to be present as a single gene and mapped to a region on chromosome 2 near two loci affecting anthocyanin pigmentation, are and aw. DFR was expressed in both leaf and hypocotyl tissue. Sequencing data from two DFR cDNA clones indicated there are alternative polyadenylation sites on DFR.
从番茄下胚轴中鉴定出一个编码二氢黄酮醇4-还原酶的cDNA克隆（DFR）。与矮牵牛、金鱼草和玉米的DFR序列进行核苷酸和氨基酸序列比较，证实该cDNA编码DFR结构基因。在番茄中，DFR序列似乎以单基因形式存在，并定位到2号染色体上靠近两个影响花青素色素沉着的基因座are和aw的区域。DFR在叶片和下胚轴组织中均有表达。来自两个DFR cDNA克隆的测序数据表明，DFR上存在多个聚腺苷酸化位点。
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