Faibra D T
Laboratoire de Farcha, Ndjaména, Tchad.
Rev Elev Med Vet Pays Trop. 1993;46(1-2):253-6.
There is evidence of antigenic diversity and of differences in virulence in Dermatophilus congolensis. For the understanding of the epidemiology of dermatophilosis it is important to distinguish between strains of the organism. Twenty field isolates from cattle in Chad and Cameroon, and an American reference strain, have been examined on restriction fragment length polymorphisms. After restriction enzyme digestion of DNA by BamHI and Southern blotting, a rDNA probe consisting of plasmid pMC5 carrying a 4.8 kb insert of Mycoplasma capricolum DNA coding for the 5S, 23S and part of 16S rRNA allowed to distinguish 6 ribotypes of D. congolensis, based on their hybridized rDNA patterns. Particular ribotypes may be distributed over a wide geographical area. On the other hand, strains belonging to at least 5 different ribotypes may be found in one herd; this may partly explain the lack of success in immunization against dermatophilosis in the field.
有证据表明刚果嗜皮菌存在抗原多样性和毒力差异。为了解嗜皮菌病的流行病学，区分该生物体的菌株很重要。对来自乍得和喀麦隆牛的20个野外分离株以及一个美国参考菌株进行了限制性片段长度多态性检测。用BamHI对DNA进行限制性酶切并进行Southern印迹后，一个由携带4.8 kb山羊支原体DNA插入片段（编码5S、23S和部分16S rRNA）的质粒pMC5组成的rDNA探针，基于其杂交的rDNA模式，可区分出刚果嗜皮菌的6种核糖型。特定的核糖型可能分布在广泛的地理区域。另一方面，在一个牛群中可能发现至少属于5种不同核糖型的菌株；这可能部分解释了在野外针对嗜皮菌病免疫接种未取得成功的原因。
