Deb G, Dominici C, Angioni A, Balloni P, Catena G, Fidani P, De Sio L, Landolfo A, Amici A, Helson L
Pediatric Oncology Services, Ospedale Bambino Gesu, Rome, Italy.
Anticancer Res. 1993 Nov-Dec;13(6B):2569-71.
A method for collecting peripheral blood mononuclear cells following mobilizing chemotherapy in pediatric patients is described. The critical elements of the method included temporary heparinization of the patient to reduce citrate overload, and limiting extracorporeal circulation to 15% of the patient's blood volume using packed red blood cells and albumin. A median of 0.9 x 10(8) mononuclear cells/kg per collection were harvested in 40 collections from eight patients with only one episode of fever and chills. Peripheral blood stem cells were reinfused into six of these patients with refractory/recurrent pediatric tumors after intensive chemotherapy. Bone marrow reconstitution followed with a mean of 30 days (19-38) for absolute neutrophils and 48 days (32-275+) for platelets. Previous chemotherapy did not appear to affect peripheral blood stem cell efficacy in reconstituting chemotherapy-ablated bone marrow.
描述了一种在儿科患者动员化疗后采集外周血单个核细胞的方法。该方法的关键要素包括对患者进行临时肝素化以减少柠檬酸盐过载，以及使用浓缩红细胞和白蛋白将体外循环限制在患者血容量的15%。在对8名患者进行的40次采集过程中，每次采集的单个核细胞中位数为0.9×10⁸个/千克，仅出现1次发热和寒战。外周血干细胞在强化化疗后被回输到其中6名患有难治性/复发性儿科肿瘤的患者体内。中性粒细胞绝对值平均在30天（19 - 38天）后实现骨髓重建，血小板则在48天（32 - 275 +天）后实现骨髓重建。先前的化疗似乎并未影响外周血干细胞在重建化疗所致骨髓消融方面的疗效。
