Yamada G, Kioussi C, Schubert F R, Eto Y, Chowdhury K, Pituello F, Gruss P
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Germany.
Biochem Biophys Res Commun. 1994 Mar 15;199(2):552-63. doi: 10.1006/bbrc.1994.1264.
Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.
胚胎干细胞（ES）可用于分析细胞分化的体外机制。我们利用ES细胞来源的胚状体形成来研究体外分化产生的细胞类型。为此，使用了多种分子标记，最好是那些与小鼠胚胎发育过程中的调控事件相关的分子标记。具体而言，使用了短尾型（T）、Pax-3和Pax-6基因以及Nkx-1.1。我们能够证明这些基因在体外的表达受诸如激活素A或碱性成纤维细胞生长因子（bFGF）等生长因子的调控。本文讨论了这些发现的意义以及鉴定新基因的可能应用。
