Nakahara Y, Takahashi K, Konuma K
National Institute of Hygienic Sciences, Tokyo, Japan.
Forensic Sci Int. 1993 Dec;63(1-3):109-19. doi: 10.1016/0379-0738(93)90265-c.
The excretion of methoxyphenamine (MOP) and methamphetamine (MA) into beards has been studied. Six healthy male subjects orally took 50 mg of MOP at a single dose and 7 doses for a successive 7 days. Their beard hairs were collected by an electric shaver every morning until MOP disappeared from the beard. After washing with 0.1% SDS, the beard samples were extracted with methanol-5 N HCl (20:1) under ultra-sonication for 1 h and the solution was kept overnight. MOP in the extract was determined by GC/MS using deuterium labelled MOP as an internal standard after trifluoroacetyl-derivatization. The drug concentrations in beard and the reproducibility of analysis were compared with the three procedures, unwashed, 0.1% SDS (wash I) and the additional ethanol (wash II) wash. The drug concentration in beard after SDS wash was 0.5-2.5 ng/mg lower than that in unwashed beard during the first 5-6 days. The drug concentration in beard after ethanol wash was much lower than that in the unwashed beard. The drug excreted into beard was detected 10 approximately 12 days for a single dose and 12-14 days for 7 doses after the last dosage at the cut off level of 1 ng/mg. On the contrary, the drug excreted in urine was not detected after more than 3 days after use. O-Desmethyl MOP, a major metabolite of MOP, was also detected in beard. The procedures were applied to the detection of MA in beard of MA abusers. It was realized that a beard sample was more useful than a urine sample assuming a longer detection.
已对甲氧苯丙胺（MOP）和甲基苯丙胺（MA）在胡须中的排泄情况进行了研究。6名健康男性受试者单次口服50毫克MOP，并连续7天每天服用7剂。每天早晨用电动剃须刀收集他们的胡须，直到胡须中检测不到MOP。用0.1%十二烷基硫酸钠（SDS）洗涤后，将胡须样本在超声处理下用甲醇 - 5N盐酸（20:1）提取1小时，溶液保存过夜。提取液中的MOP在经三氟乙酰衍生化后，以氘标记的MOP作为内标，通过气相色谱 - 质谱联用（GC/MS）进行测定。将未洗涤、0.1% SDS（洗涤I）和额外乙醇（洗涤II）洗涤这三种方法用于比较胡须中的药物浓度及分析的重现性。在前5 - 6天，SDS洗涤后胡须中的药物浓度比未洗涤胡须中的低0.5 - 2.5纳克/毫克。乙醇洗涤后胡须中的药物浓度比未洗涤胡须中的低得多。在末次给药后，单剂量给药时，胡须中排泄的药物在大约10至12天被检测到，7剂给药时在12 - 14天被检测到，检测下限为1纳克/毫克。相反，用药后3天以上尿液中未检测到排泄的药物。MOP的主要代谢产物O - 去甲基MOP也在胡须中被检测到。该方法被应用于检测甲基苯丙胺滥用者胡须中的MA。结果发现，假设检测时间更长，胡须样本比尿液样本更有用。
