一种含有与DRB1*0103等位基因相关的DRB5基因的新型HLA单倍型。

A novel HLA haplotype containing a DRB5 gene associated with the DRB1*0103 allele.

作者信息

Perrier P, Leotard B, Henry M A

机构信息

Laboratoire d'Histocompatibilité, Centre Régional de Transfusion Sanguine, Vandoeuvre les Nancy, France.

出版信息

Tissue Antigens. 1993 Nov;42(5):518-21. doi: 10.1111/j.1399-0039.1993.tb02197.x.

DOI:10.1111/j.1399-0039.1993.tb02197.x

PMID:7908467

Abstract

A new hybrid haplotype, a DRB10103 allele associated with a DRB50101 allele, was found in a French Caucasoid family and has been described here. When these cells were typed by serology, contrarily to cells with the DR1 + 2s haplotype, they did not seem to be triplets. The reactivity of these cells with the DR2 allosera led to a false serological HLA-DR typing. RFLP analysis and DNA oligotyping after DR1-DRB1, DR2-DRB1 and DRB5 group-specific amplifications showed that there was no DR2-DRB1 product in these cells and demonstrated the segregation of a DR103 DR51 haplotype in the family.

摘要

在一个法裔高加索家庭中发现了一种新的杂合单倍型，即与DRB50101等位基因相关的DRB10103等位基因，并在此进行了描述。当通过血清学对这些细胞进行分型时，与具有DR1 + 2s单倍型的细胞相反，它们似乎不是三联体。这些细胞与DR2同种异体血清的反应性导致了错误的血清学HLA-DR分型。在DR1-DRB1、DR2-DRB1和DRB5组特异性扩增后的RFLP分析和DNA寡核苷酸分型显示，这些细胞中没有DR2-DRB1产物，并证明了该家族中DR103 DR51单倍型的分离。