使用市售检测方法检测白细胞中的HIV-1 DNA。

Detection of HIV-1 DNA in leukocytes using a commercially available assay.

作者信息

Zaaijer H L, Cuypers H T, Reesink H W, ten Veen J H, Schoon-Visser S C, Lelie P N

机构信息

Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.

出版信息

Vox Sang. 1994;66(1):78-80. doi: 10.1111/j.1423-0410.1994.tb00282.x.

DOI:10.1111/j.1423-0410.1994.tb00282.x

PMID:7908474

Abstract

Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection.

摘要

最近，一种用于检测白细胞中HIV-1前病毒DNA的检测方法开始商业化应用。这种检测方法（Amplicor HIV-1检测，罗氏诊断系统公司）利用聚合酶链反应将HIV-1 DNA扩增至可检测水平。我们对74份来自HIV-1感染患者的样本和41份来自健康献血者的样本进行了该检测方法的性能研究。有两次阴性对照样本出现了错误的阳性反应。然而，对患者样本和献血者样本的敏感性和特异性均为100%。为避免出现假阳性结果，我们建议如果没有其他数据证实HIV感染，应对最初呈阳性反应的样本进行重复检测。