Liechti-Gallati S, Borer U
Aus dem Institut für Rechtsmedizin der Universität Bern.
Arch Kriminol. 1994 Jan-Feb;193(1-2):37-42.
Some of the commonly used presumptive test reagents for identification of blood and semen could affect the recovery or the analysis of high-molecular-weight DNA from evidentiary samples. The study presents data on restriction fragment length polymorphisms (RFLP) and polymerase chain reaction (PCR) analyses from blood and semen stains treated with Hemastix (Bayer Diagnostics), Sangur (Boehringer) and Phosphatesmo-KM-paper (Macherey-Nagel). The results demonstrate that the three tests examined do not have any effect on quality and quantity of the DNA nor on DNA typing by RFLP and PCR analyses. In conclusion, presumptive testing of blood and semen stains using Hemastix, Sangur, or Phosphatesmo paper can principally be carried out prior to submission to DNA analysis. In spite of these findings, it is recommended to continue the prudent practice of testing only small portions of an evidentiary stain in order to prevent contamination.
一些常用于鉴定血液和精液的推定测试试剂可能会影响从证据样本中回收或分析高分子量DNA。该研究展示了用Hemastix（拜耳诊断公司）、Sangur（勃林格公司）和Phosphatesmo-KM试纸（默克密理博公司）处理过的血液和精液污渍的限制性片段长度多态性（RFLP）和聚合酶链反应（PCR）分析数据。结果表明，所检测的这三种测试对DNA的质量和数量以及通过RFLP和PCR分析进行的DNA分型均无任何影响。总之，使用Hemastix、Sangur或Phosphatesmo试纸对血液和精液污渍进行推定测试原则上可在提交DNA分析之前进行。尽管有这些发现，但建议继续谨慎行事，仅对一小部分证据污渍进行测试，以防止污染。
