Olsen J, Laustsen L, Troelsen J
Department of Medical Biochemistry, Panum Institute, University of Copenhagen, Denmark.
FEBS Lett. 1994 Apr 11;342(3):325-8. doi: 10.1016/0014-5793(94)80525-3.
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.
利用Caco-2细胞系研究了肝细胞核因子1（HNF1）结合蛋白对肠氨肽酶N表达的重要性。Caco-2细胞中的氨肽酶N启动子活性依赖于HNF1元件（位置-85至-58），与HNF1α表达载体共转染表明HNF1α通过该元件直接激活启动子。使用Caco-2细胞核提取物进行的电泳迁移率变动分析表明，无论其分化状态如何，都存在大量HNF1结合蛋白。
