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假孕通过上皮启动子诱导兔子宫内膜中肝细胞核因子-1β及其靶基因氨肽酶N的表达。

Pseudopregnancy induces the expression of hepatocyte nuclear factor-1 beta and its target gene aminopeptidase N in rabbit endometrium via the epithelial promoter.

作者信息

Olsen J, Classen-Linke I, Sjöström H, Norén O

机构信息

Department of Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark.

出版信息

Biochem J. 1995 Nov 15;312 ( Pt 1)(Pt 1):31-7. doi: 10.1042/bj3120031.

DOI:10.1042/bj3120031
PMID:7492330
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136223/
Abstract

The rabbit endometrium is an excellent model system allowing experimental manipulation of aminopeptidase N (APN) mRNA expression in vivo. By RNase mapping and sequencing of cloned PCR-amplified primer-extended RNA, it was demonstrated that endometrial APN expression is directed by the epithelial APN promoter and is increased in human-choriogonadotropin-induced pseudopregnancy. Cloning and sequencing of the rabbit APN epithelial promoter revealed conservation of the upstream footprint (UF), hepatocyte nuclear factor-1 (HNF1) and Sp1 elements known to be present in the pig and human promoters as well. The pseudopregnancy-induced APN expression was found to be accompanied by a parallel increase in the level of the transcription factor HNF1 beta, whereas a much smaller increase in Sp1 and UF-binding proteins was observed. This indicates that HNF1 beta acts as a switch triggering the pregnancy-induced APN expression. The sequence of the UF element suggests members of the nuclear hormone-receptor superfamily as possible UF-binding proteins, and competition experiments suggest that the chicken ovalbumin upstream promoter transcription factor functions as such in the rabbit endometrium.

摘要

兔子宫内膜是一个出色的模型系统，可在体内对氨肽酶N（APN）mRNA表达进行实验性调控。通过对克隆的PCR扩增引物延伸RNA进行核糖核酸酶图谱分析和测序，结果表明，子宫内膜APN表达由上皮APN启动子指导，且在人绒毛膜促性腺激素诱导的假孕过程中增加。兔APN上皮启动子的克隆和测序显示，其上游足迹（UF）、已知存在于猪和人启动子中的肝细胞核因子-1（HNF1）和Sp1元件具有保守性。研究发现，假孕诱导的APN表达伴随着转录因子HNF1β水平的平行升高，而Sp1和UF结合蛋白的升高幅度则小得多。这表明HNF1β作为一个开关触发了妊娠诱导的APN表达。UF元件的序列表明核激素受体超家族成员可能是UF结合蛋白，竞争实验表明鸡卵清蛋白上游启动子转录因子在兔子宫内膜中发挥这样的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/e1ee6180882f/biochemj00051-0043-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/824455ad394a/biochemj00051-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/e1722f8ab43b/biochemj00051-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/1687139f4d98/biochemj00051-0042-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/4dfe04e3c198/biochemj00051-0042-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/9cb85a83b498/biochemj00051-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/5d8dc521eecb/biochemj00051-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/e1ee6180882f/biochemj00051-0043-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/824455ad394a/biochemj00051-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/e1722f8ab43b/biochemj00051-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/1687139f4d98/biochemj00051-0042-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/4dfe04e3c198/biochemj00051-0042-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/9cb85a83b498/biochemj00051-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/5d8dc521eecb/biochemj00051-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3639/1136223/e1ee6180882f/biochemj00051-0043-c.jpg

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