Helmuth R, Schroeter A
Robert von Ostertag-Institute, Institute of Veterinary Medicine, Berlin, Germany.
Int J Food Microbiol. 1994 Jan;21(1-2):69-77. doi: 10.1016/0168-1605(94)90201-1.
The predominance of certain phage types of Salmonella enteritidis in various countries makes further epidemiological subgrouping necessary. Today this is achieved by using molecular typing methods. For various bacterial species, plasmid profiling, the pattern of outer membrane proteins and lipopolysaccharides, the fingerprinting of total genomic DNA including ribotyping, and multilocus enzyme electrophoretic typing, have proven very useful. When such methods have been applied to S. enteritidis, they revealed a homogeneous, clonal structure in contemporary PT4 isolates. Furthermore they indicate that the clone observed today emerged from a heterogeneous population before the onset of the epidemic.
肠炎沙门氏菌某些噬菌体类型在不同国家的优势地位使得进一步进行流行病学亚分组成为必要。如今，这可通过使用分子分型方法来实现。对于各种细菌物种，质粒图谱分析、外膜蛋白和脂多糖模式、包括核糖体分型在内的全基因组DNA指纹图谱分析以及多位点酶电泳分型已被证明非常有用。当这些方法应用于肠炎沙门氏菌时，它们揭示了当代PT4分离株中存在均匀的克隆结构。此外，这些方法表明，如今观察到的克隆是在疫情爆发前从异质群体中出现的。
