在良性和恶性胃肠道黏膜中产生岩藻糖基转移酶的唾液酸化Le(a)和唾液酸化Le(x)碳水化合物抗原

Fucosyltransferase-producing sialyl Le(a) and sialyl Le(x) carbohydrate antigen in benign and malignant gastrointestinal mucosa.

作者信息

Dohi T, Hashiguchi M, Yamamoto S, Morita H, Oshima M

机构信息

Division of Biochemistry and Nutrition, Research Institute, Tokyo, Japan.

出版信息

Cancer. 1994 Mar 15;73(6):1552-61. doi: 10.1002/1097-0142(19940315)73:6<1552::aid-cncr2820730605>3.0.co;2-6.

DOI:10.1002/1097-0142(19940315)73:6<1552::aid-cncr2820730605>3.0.co;2-6

PMID:7908856

Abstract

BACKGROUND

Sialyl Le(a) antigen and sialyl Le(x) antigen are cancer-associated carbohydrate antigens. Previous immunohistologic and immunochemical studies have shown that these antigens are preferentially expressed in gastric cancer and colonic cancer and that they possibly are related to the metastatic potential of the cancer cells. The biosynthesis of these antigens is completed by fucosyltransferases, but it has not been reported how fucosyltransferases control the expression of these carbohydrate antigens concerning the invasive potential of the cancer.

METHODS

The authors established an assay system for measuring the activity of alpha 1-->4 fucosyltransferase (sialyl Le(a) synthase) and alpha 1-->3 fucosyltransferase (sialyl Le(x) synthase) with a high-pressure liquid chromatography system (HPLC). The activity was measured in various parts of normal and cancerous gastric and colonic tissue and compared with the expression of sialyl Le(a), sialyl Le(x), Le(a), and Le(x) antigens determined in a solid-phase enzyme-linked immunosolvent assay (EIA).

RESULTS

Sialyl Le(a) synthase was detected in most normal or malignant mucosa of gastric and colonic tissues, regardless of anatomic locations. Sialyl Le(x) synthase activity generally was low in the normal gastric mucosa, whereas the activity was higher in 77% (7 of 9) of gastric cancer tissues than in corresponding normal tissues with enhanced expression of sialyl Le(x) antigen in most patients (5 of 7). In the large intestine, the activity of sialyl Le(a) synthase and sialyl Le(x) synthase was correlated. Although enhanced expression of sialyl Le(x) in colonic cancer was observed in 86% (12 of 14) of all patients, concomitant higher sialyl Le(x) synthase activity than that in normal tissue was observed in only 58% (7 of 12) of patients.

CONCLUSIONS

The expression of sialyl Le(a) and sialyl Le(x) antigens in the stomach and the colon was not controlled solely by fucosyltransferases but by a more complicated system involving other glycosyltransferases.

摘要

背景

唾液酸化Le(a)抗原和唾液酸化Le(x)抗原是与癌症相关的碳水化合物抗原。先前的免疫组织学和免疫化学研究表明，这些抗原在胃癌和结肠癌中优先表达，并且它们可能与癌细胞的转移潜能有关。这些抗原的生物合成由岩藻糖基转移酶完成，但尚未有关于岩藻糖基转移酶如何控制这些碳水化合物抗原与癌症侵袭潜能相关表达的报道。

方法

作者建立了一种用高压液相色谱系统（HPLC）测量α1→4岩藻糖基转移酶（唾液酸化Le(a)合酶）和α1→3岩藻糖基转移酶（唾液酸化Le(x)合酶）活性的检测系统。在正常和癌性胃及结肠组织的各个部位测量活性，并与在固相酶联免疫吸附测定（EIA）中测定的唾液酸化Le(a)、唾液酸化Le(x)、Le(a)和Le(x)抗原的表达进行比较。

结果

无论解剖位置如何，在胃和结肠组织的大多数正常或恶性黏膜中均检测到唾液酸化Le(a)合酶。唾液酸化Le(x)合酶活性在正常胃黏膜中通常较低，而在77%（9例中的7例）的胃癌组织中活性高于相应的正常组织，大多数患者（7例中的5例）唾液酸化Le(x)抗原表达增强。在大肠中，唾液酸化Le(a)合酶和唾液酸化Le(x)合酶的活性相关。尽管在所有患者的86%（14例中的12例）中观察到结肠癌中唾液酸化Le(x)表达增强，但仅在58%（12例中的7例）的患者中观察到与正常组织相比唾液酸化Le(x)合酶活性更高。