Kato T, Terui T, Zhen Y X, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Exp Dermatol. 1993 Aug;2(4):171-4. doi: 10.1111/j.1600-0625.1993.tb00028.x.
Stratum corneum (SC) exerts a proinflammatory effect in the presence of complement. When Staphylococcus aureus (S. aureus) invades the skin through damaged SC, neutrophils accumulate at the subcorneal portion of epidermis to phagocytize the S. aureus as noted in impetigo. Besides the phagocytosis of bacteria, neutrophils interact with opsonized SC in a form of frustrated phagocytosis, increasing a damage of the surrounding tissues. Based on our previous finding that staphylococcal protein A promotes the interaction between SC and neutrophils, we investigated whether lipoteichoic acid (LTA), another cell wall component of S. aureus, also shows similar properties. We found that LTA significantly promoted the binding of neutrophils to opsonized SC, resulting in an increase in SC-induced respiratory burst of neutrophils assessed by chemiluminescence (CL). The binding of neutrophils to the SC was almost completely inhibited by the blocking of CR3 with anti-CD11b antibody, suggesting that the binding between SC and neutrophils is mediated by interaction between C3bi and CR3 (Mac-1). Such enhanced interaction seems to function in the primary host defence mechanism against the invading S. aureus through the skin such as in impetigo.
角质层（SC）在补体存在的情况下会产生促炎作用。当金黄色葡萄球菌（S. aureus）通过受损的SC侵入皮肤时，如脓疱病中所见，中性粒细胞会在表皮的角膜下部分积聚以吞噬金黄色葡萄球菌。除了吞噬细菌外，中性粒细胞还以受挫吞噬的形式与调理过的SC相互作用，增加周围组织的损伤。基于我们之前的发现，即葡萄球菌蛋白A促进SC与中性粒细胞之间的相互作用，我们研究了金黄色葡萄球菌的另一种细胞壁成分脂磷壁酸（LTA）是否也具有类似特性。我们发现LTA显著促进中性粒细胞与调理过的SC的结合，导致通过化学发光（CL）评估的SC诱导的中性粒细胞呼吸爆发增加。用抗CD11b抗体阻断CR3几乎完全抑制了中性粒细胞与SC的结合，这表明SC与中性粒细胞之间的结合是由C3bi与CR3（Mac-1）之间的相互作用介导的。这种增强的相互作用似乎在针对金黄色葡萄球菌通过皮肤入侵的主要宿主防御机制中起作用，如在脓疱病中。
