Demeter L M, Stoler M H, Broker T R, Chow L T
Department of Medicine, University of Rochester School of Medicine and Dentistry, NY.
Hum Pathol. 1994 Apr;25(4):343-8. doi: 10.1016/0046-8177(94)90141-4.
The expression of proliferating cell nuclear antigen (PCNA) was studied in human papillomavirus (HPV)-infected, benign and malignant lesions of the genital tract and larynx using immunocytochemical staining of formalin-fixed clinical specimens. We observed the induction of PCNA in squamous carcinomas and adenocarcinomas, as has been demonstrated with other malignancies. In addition, the differentiated keratinocytes of the upper spinous cells and granulocytes in condylomata acuminata and low-grade intraepithelial neoplasias showed a consistent induction of PCNA compared with the normal squamous epithelium, in which only some of the parabasal and basal cells were positive. This reactivation of PCNA synthesis correlated with the presence of high copy numbers of HPV DNA and was independent of the oncogenic risk potential of the infecting HPV genotype. We postulate that HPV gene products induce the expression of PCNA and other components of the host DNA replication machinery in differentiated cells of squamous lesions to facilitate vegetative viral replication.
利用福尔马林固定的临床标本进行免疫细胞化学染色，研究了增殖细胞核抗原（PCNA）在人乳头瘤病毒（HPV）感染的生殖道和喉部良性及恶性病变中的表达情况。我们观察到，正如在其他恶性肿瘤中所证实的那样，鳞状细胞癌和腺癌中PCNA被诱导表达。此外，与正常鳞状上皮相比，尖锐湿 疣和低级别上皮内瘤变中的棘层上部细胞和颗粒细胞的分化角质形成细胞显示出PCNA的持续诱导表达，在正常鳞状上皮中只有一些基底旁细胞和基底细胞呈阳性。PCNA合成的这种重新激活与高拷贝数HPV DNA的存在相关，并且与感染的HPV基因型的致癌风险潜力无关。我们推测，HPV基因产物在鳞状病变的分化细胞中诱导PCNA及宿主DNA复制机制的其他成分的表达，以促进病毒的增殖性复制。
